Puromycin Nacetyltransferase | Puromycin Pac Marker

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Puromycin structure and mechanism of action. (A) Chemical structures of the 3′ end of an aminoacylated tyrosyl-tRNA (left) and puromycin (right).The different bonds between the

A novel positive/negative selection cassette, puDeltatk, was generated. pu(Delta)tk is a bifunctional fusion protein between puromycin N-acetyltransferase (Puro) and

PPT - Lectures 33-34 GENETIC CODE and PROTEIN SYNTHESIS PowerPoint ...

OPEN Structure‑guided selection of puromycin N

In this study, we describe the puromycin-N-acetyltransferase (PAC) gene isolated from Streptomyces alboninger as a new selectable marker to obtain stable transfection in P.

Puromycin and the Streptomyces alboniger‐derived puromycin N‐acetyltransferase (PAC) enzyme form a commonly used system for selecting stably transfected cultured cells. The crystal

De Maio A., Buchman T. G. Mammalian liver contains an activity which mimics bacterial chloramphenicol acetyltransferase. Biochim Biophys Acta. 1990 Nov 30;1087 (3):303–308. doi:

Puromycin resistance gene as an effective selection marker for ciliate
7K09: Puromycin N-acetyltransferase in complex with acetyl-CoA
Anti-Human Puromycin N-acetyltransferase monoclonal antibody

A gene (pac) encoding a puromycin N-acetyl transferase (PAC) of Streptomyces alboniger ATCC12461 was cloned in the Streptomyces plasmid pIJ702 and expressed in S. lividans

Puromycin and its derivative O-propargyl puromycin (OPP) have recently found widespread use in detecting nascent proteins. Use of these metabolic labels in complex mixtures of cells leads to indiscriminate tagging of

Puromycin is produced by Streptomyces alboniger, a gram-positive actinomycete, through a series of enzymatic reactions starting from adenosine triphosphate (ATP)

Recombinant Puromycin N-acetyltransferase protein

Puromycin-N-acetyltransferase as a selectable marker for use in Plasmodium falciparum Mol Biochem Parasitol. 2001 Oct;117(2):155-60. doi: 10.1016/s0166-6851(01)00344-9. Authors T F

A puromycin-N-acetyltransferase gene (pac) is widely used as a selection marker for eukaryotic gene manipulation. However, it has never been utilized for molecular studies in

Like other antibiotic-producing Streptomyces, S. alboniger ribosomes are sensitive to their own antibiotic. 19 Puromycin resistance is mediated by a puromycin acetyltransferase (PAC) that

In this paper, we demonstrate that P. falciparum is highly sensitive to the drug puromycin, but that transgenic expression of the puromycin-N-acetyltransferase (PAC) gene from Streptomyces

Resistance to puromycin is encoded by puromycin N-acetyltransferase (PAC), which is also expressed by S. alboniger. pac gene was first cloned in 1985 and used as

Puromycin and the Streptomyces alboniger-derived puromycin N-acetyltransferase (PAC) enzyme form a commonly used system for selecting stably transfected cultured cells.

Puromycin N-acetyltransferase also known as PAC is an enzyme with a molecular mass of approximately 23 kDa. It catalyzes the acetylation of puromycin an antibiotic which inactivates

Puromycin and the Streptomyces alboniger-derived puromycin N-acetyltransferase (PAC) enzyme form a commonly used system for selecting stably transfected cultured cells. The crystal structure of PAC has been solved

Puromycin dihydrochloride. Puromycin dihydrochloride is a aminonuclease antibiotic that inhibits protein synthesis. Puromycin is used for selection and maintenance of cell lines expressing a

Here, we show how a widely used mammalian selection marker, puromycin N-acetyltransferase, can be repurposed for cell-specific metabolic labeling. This approach, which we named

The resulting fusion protein (EGFP-puro) confers both green fluorescence and resistance to puromycin when expressed in mammalian cells. How to cite this plasmid ( Back to top) These

Moreover, the puromycin N-acetyltransferase (PAC) gene from Streptomyces alboniger encodes a small protein which provides resistance to the antibiotic. This suggested that a codon

Furthermore, a variety of artifacts and side effects, resulting from the sequences within the plasmid vector backbone, application of cytotoxic transfection chemical reagents

This codon-optimized pac gene expressed in T. thermophila conferred a resistance to transformed cells against 2,000 μg/ml of puromycin dihydrochloride, whereas the

In this study, we describe the puromycin-N-acetyltransferase (PAC) gene isolated from Streptomyces alboninger as a new selectable marker to obtain stable transfection in P.

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